NCT07271381 | RECRUITING | Allergic

Detailed Description:

Study Description: In this study, we aim to investigate whether there is a link between lifestyle exposures, inflammation, and disease by exposing participants to environments with real-world levels of common skin and hair products (eg, soaps, shampoos), a typical Western diet of processed food, and/or ubiquitous air exposures, and to environments with cleaner, more natural contactants and less processed food. Participants will spend up to 7 days as an inpatient in a controlled environment with the assigned exposures, and up to 7 days as an inpatient in a cleaner environment. We will collect a variety of biospecimens and data throughout the inpatient stays to identify changes in skin, gastrointestinal (GI), and airway survey parameters. The study will consist of 2 stages with a crossover design in each stage. Stage 1 will be divided into 3 cohorts: one cohort to identify changes in skin, one for GI changes, and one for airway changes. Within each cohort, participants will be block randomized to spend up to 7 days in the inpatient unit with either the specifically assigned experimental (common) or control (pure) exposures. Within each of the 3 cohorts, the common exposures will target a specific organ system (skin, GI, or airway). After a brief washout period, participants will then crossover to the other targeted exposure (ie, from common to pure or vice versa), for an additional inpatient stay of up to 7 days. The findings from each participant s first inpatient stay will be compared to findings from their crossover second inpatient stay. The data collected from Stage 1 may be used to clarify the survey parameters of participants enrolled into Stage 2, in which new participants will be randomized to all common exposure domains (skin + GI + airway) vs. pure (control) and then a crossover. Objectives: Stage 1 Primary Objectives: 1. Cohort 1: Determine the effects of common vs. pure exposures on the skin in healthy volunteers. 2. Cohort 2: Determine the effects of common vs. pure exposures on the GI tract in healthy volunteers. 3. Cohort 3: Determine the effects of common vs. pure exposures on the airway in healthy volunteers. Stage 1 Secondary Objective: Determine the combined effects of common vs. pure exposures on the skin, gut, and airway in healthy volunteers. Stage 2 Primary Objective: Determine the combined effects of common vs. pure exposures on the skin, gut, and airway in healthy volunteers. Endpoints: Stage 1 Primary Endpoints: 1. Cohort 1 (skin): The primary endpoint for the skin substudy is the skin impedance change response from the common environment (as a log geometric mean ratio \[GMR\]) minus the mean skin impedance change response from the pure environment (as a log GMR). For each crossover intervention period (common or pure), we measure the GMR as the ratio of the geometric mean (GM) of the average skin impedance measurements at the last day of the inpatient stay over the GM of the average skin impedance measurements at day 0 (baseline). 2. Cohort 2 (GI): The primary endpoint for the GI substudy is analogous to that of the skin substudy, except replacing skin impedance with the Shannon-Weaver diversity index (a measure of metabolic diversity) measured on the gut microbiome. 3. Cohort 3 (airway): The primary endpoint for the airway substudy is the airway maximum change response from the common environment (as the log of the GM of the maximum of the daily change response) minus the airway maximum change response from the pure environment. For each crossover intervention period (common or pure), we measure each individual s daily change response as airway resistance (R5) measured at each day of the inpatient stay over the R5 at day 0 (baseline). Stage 1 Secondary Endpoints will compare common vs pure crossover period responses as follows: 1. Cohort 1 (skin): * Proportion of participants that experience a 30% reduction in skin impedance from admission to any time point during inpatient stay. * Change in metabolic functional analysis and/or specific taxa of skin microbiome from admission to last day of the inpatient stay. * Change in skin metabolomics by tape strip analysis during study exposure. 2. Cohort 2 (GI): * Change in metabolic functional analysis and/or specific taxa of gut microbiome. * Change in diversity index, metabolic functional analysis, and/or specific taxa of oral microbiome. 3. Cohort 3 (airway): \- Increase in R5 from admission to last day of inpatient stay by impulse oscillometry (IOS). 4. All 3 Cohorts: * Change in serum and/or intradermal fluid inflammatory markers during study exposure. * Change in serum lipopolysaccharides (LPS) and bacterial translocation markers (a marker of GI barrier integrity) during study exposure. * Change in quality of life from baseline during exposures to contactants. * Changes in activity from baseline during study exposure. * Change in sense of smell/taste during study exposure. * Correlation of exposures with activity and/or symptoms. Stage 2 Endpoint: Based on isolated exposures from single organ systems and randomization design in Stage 1, repeat primary and secondary endpoint responses of Stage 1.