University of Copenhagen
Jorgen FP Wojtaszewski
The study investigates the role of one single bout of exercise in mediating enhancement of muscle insulin sensitivity following a single bout of exercise. Furthermore, the study is aiming to elucidate the temporal development in insulin signaling, at the early timepoints of insulin stimulation that may be responsible for the enhanced muscle insulin sensitivity. This will be investigated in young healthy males subjected to a one-legged knee-extensor exercise followed by a hyperinsulinemic-euglycemic clamp, a setup known to enhance muscle insulin sensitivity.
Insulin Sensitivity
One-legged-knee extensor exercise
Insulin infusion
Muscle Biopsy Medication: Lidocaine
Exercise
NA
The participant arrives fasting at 8:00 AM, having abstained from alcohol, nicotine, and caffeine for the past 24 hours and refrained from strenuous physical activity for the past 48 hours. The participants are then subjected to a one-legged knee extension exercise performed by the participant from 9:00 AM to 10:00 AM. Groin catheters are inserted into the femoral artery and vein using ultrasound guidance and sterile technique Two hours after the completion of the exercise, a catheter is inserted into an arm vein (antecubital vein) and groin catheters are inserted into the femoral artery and vein using ultrasound guidance and sterile technique. Four hours after the exercise ends, a hyperinsulinemic-euglycemic clamp is commenced. The clamp begins with a bolus injection of insulin, followed by a continuous infusion. Glucose is infused concurrently to maintain constant blood glucose levels. Throughout the procedure, blood samples are collected every 5 minutes from the femoral catheters, and blood flow is measured using ultrasound Doppler. Muscle biopsies from the vastus lateralis of both legs are taken before the clamp starts and at 3, 6, 10, and 20 minutes after the clamp's initiation. Following the 20-minute mark, the clamp continues until steady-state is achieved to determine tissue-specific and whole-body insulin sensitivity. Over the course of the experiment, a total of 10 muscle biopsies and approximately 150 mL of blood are collected. The experimental part of the study is expected to conclude by 4:30 PM. Participants are provided with food and beverages and observed for 1 hour before being allowed to leave the research laboratory.
| Study Type : | INTERVENTIONAL |
| Estimated Enrollment : | 8 participants |
| Masking : | NONE |
| Primary Purpose : | BASIC_SCIENCE |
| Official Title : | The Significance of Physical Activity for Insulin Signaling Dynamics and Insulin Sensitivity |
| Actual Study Start Date : | 2025-05-05 |
| Estimated Primary Completion Date : | 2026-12-15 |
| Estimated Study Completion Date : | 2027-01 |
Information not available for Arms and Intervention/treatment
| Ages Eligible for Study: | 22 Years to 35 Years |
| Sexes Eligible for Study: | MALE |
| Accepts Healthy Volunteers: | 1 |
Want to participate in this study, select a site at your convenience, send yourself email to get contact details and prescreening steps.
RECRUITING
University of Copenhagen, August Krogh Section for Human and Molecular Physiology
Copenhagen, Denmark, 2100